Constitutive nitric oxide synthase-like enzyme in two species involved in cutaneous and mucocutaneous leishmaniasis

Leishmania is an obligate intracellular parasite that primarily inhabits macrophages. The destruction of the in host cell a fundamental mechanism for infection control. In addition, inhibition leishmanicidal activity macrophages seems to be related ability some species inhibit production nitric oxide (NO) by depleting arginine. Some have produce NO from constitutive synthase-like enzyme (cNOS-like). However, localization cNOS-like has not been described before. As such, this study was designed locate and promastigotes (Leishmania) amazonensis (Viannia) braziliensis. initially quantified flow cytometry, which indicated significant difference between L. (L.) (GMFC = 92.17 +/− 4.6) (V.) braziliensis 18.89 2.29) (P < 0.05). Analysis cNOS expression immunoblotting showed more versus Subsequently, immunolabeling observed regions near vesicles, flagellar pocket mitochondria, small clusters particles appeared fusing with vesicles suggestive glycosomes, peroxisome-like-organelles compartmentalize glycolytic pathway trypanosomatid parasites. confocal microscopy analysis demonstrated colocalization GAPDH, specific marker glycosomes. Thus, produces greater amounts than braziliensis, both present inside